6URB
Pseudomonas aeruginosa HasA mutant - H32A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 291 |
| Detector technology | CCD |
| Collection date | 2019-07-25 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 111.340, 51.867, 72.811 |
| Unit cell angles | 90.00, 130.67, 90.00 |
Refinement procedure
| Resolution | 29.891 - 2.096 |
| R-factor | 0.2364 |
| Rwork | 0.233 |
| R-free | 0.30220 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ell |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.159 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.170 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Number of reflections | 17648 | 17648 |
| <I/σ(I)> | 17 | 2.9 |
| Completeness [%] | 94.0 | |
| Redundancy | 3.6 | |
| CC(1/2) | 0.995 | 0.910 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 1M Sodium Citrate, 0.1M Tris HCl pH 7.4, 0.2M Sodium Chloride |
