6UQJ
Crystal structure of the GH39 enzyme from Xanthomonas axonopodis pv. citri
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE W01B-MX2 |
Synchrotron site | LNLS |
Beamline | W01B-MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-02-21 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.459 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 117.140, 57.097, 81.120 |
Unit cell angles | 90.00, 107.53, 90.00 |
Refinement procedure
Resolution | 28.930 - 1.707 |
R-factor | 0.1641 |
Rwork | 0.162 |
R-free | 0.19710 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ekj |
RMSD bond length | 0.007 |
RMSD bond angle | 1.168 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 38.670 | 1.768 |
High resolution limit [Å] | 1.707 | 1.707 |
Number of reflections | 54724 | 5211 |
<I/σ(I)> | 20.09 | |
Completeness [%] | 98.2 | |
Redundancy | 6 | |
CC(1/2) | 0.999 | 0.848 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M MIB Buffer pH 5.0 and 25% (w/v) PEG 1500 |