6UQ1

RNA polymerase II elongation complex with 5-guanidinohydantoin lesion in state 6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9767
Spacegroup name	C 1 2 1
Unit cell lengths	170.502, 223.508, 194.943
Unit cell angles	90.00, 101.58, 90.00

Refinement procedure

Resolution	49.521 - 3.600
R-factor	0.2409
Rwork	0.240
R-free	0.28740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6blo
Data reduction software	XDS
Data scaling software	Aimless (0.7.3)
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.521	3.600
High resolution limit [Å]	3.540	3.540
Rmerge	0.491	3.695
Rmeas	0.531	3.984
Rpim	0.200	1.481
Total number of observations		33112
Number of reflections	86875	4609
<I/σ(I)>	5.1	0.6
Completeness [%]	100.0	100
Redundancy	7	7.2
CC(1/2)	0.979	0.251

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	390mM (NH4)2HPO4/NaH2PO4, pH 6.5, 5mM dioxane, 5mM DTT, 10-12% PEG6000