6UQ1
RNA polymerase II elongation complex with 5-guanidinohydantoin lesion in state 6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-23 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9767 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 170.502, 223.508, 194.943 |
| Unit cell angles | 90.00, 101.58, 90.00 |
Refinement procedure
| Resolution | 49.521 - 3.600 |
| R-factor | 0.2409 |
| Rwork | 0.240 |
| R-free | 0.28740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6blo |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.3) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.521 | 3.600 |
| High resolution limit [Å] | 3.540 | 3.540 |
| Rmerge | 0.491 | 3.695 |
| Rmeas | 0.531 | 3.984 |
| Rpim | 0.200 | 1.481 |
| Total number of observations | 33112 | |
| Number of reflections | 86875 | 4609 |
| <I/σ(I)> | 5.1 | 0.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7 | 7.2 |
| CC(1/2) | 0.979 | 0.251 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 390mM (NH4)2HPO4/NaH2PO4, pH 6.5, 5mM dioxane, 5mM DTT, 10-12% PEG6000 |
