6UQ0

RNA polymerase II elongation complex with 5-guanidinohydantoin lesion in state 4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97665
Spacegroup name	C 1 2 1
Unit cell lengths	167.897, 223.273, 193.139
Unit cell angles	90.00, 100.94, 90.00

Refinement procedure

Resolution	49.697 - 3.560
R-factor	0.2366
Rwork	0.235
R-free	0.27830
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6blo
Data reduction software	XDS
Data scaling software	Aimless (0.7.3)
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.700	3.630
High resolution limit [Å]	3.560	3.560
Rmerge	0.684	2.794
Rmeas	0.740	3.012
Rpim	0.279	1.120
Total number of observations	580773	32694
Number of reflections	83440	4552
<I/σ(I)>	3.7	0.8
Completeness [%]	100.0	100
Redundancy	7	7.2
CC(1/2)	0.933	0.303

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	390mM (NH4)2HPO4/NaH2PO4, pH 6.5, 5mM dioxane, 5mM DTT, 10-12% PEG6000