6UOW

MicroED structure of OsPYL/RCAR5 (24-29) at 12 e-/A^2

Experimental procedure

Source type	ELECTRON MICROSCOPE
Source details	Technai F30
Temperature [K]	100
Detector technology	CMOS
Collection date	2019-04-04
Detector	TemCam-XF416
Wavelength(s)	0.0197
Spacegroup name	P 21 21 21
Unit cell lengths	4.730, 11.420, 39.590
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.479 - 1.200
R-factor	0.2779
Rwork	0.269
R-free	0.35850
Data scaling software	XSCALE
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	7.479	7.479	1.500
High resolution limit [Å]	1.200	8.000	1.200
Rmerge	0.252		0.696
Rmeas	0.269		0.739
Total number of observations	6454
Number of reflections	737		339
<I/σ(I)>	3.74		2.34
Completeness [%]	90.0		90.4
Redundancy	8.757		9.386
CC(1/2)	0.976		0.812

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	10% ethanol