6UOP

OsPYL/RCAR5 (24 - 29) solved by nanobeam diffraction tomography

Experimental procedure

Source type	ELECTRON MICROSCOPE
Source details	TEAM1
Temperature [K]	100
Detector technology	CMOS CAMERA
Collection date	2019-04-04
Detector	K2 IS
Wavelength(s)	0.0197
Spacegroup name	P 21 21 21
Unit cell lengths	4.710, 11.490, 38.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	5.745 - 1.351
R-factor	0.2545
Rwork	0.253
R-free	0.25960
Data scaling software	XSCALE
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	5.745	5.745	1.560
High resolution limit [Å]	1.350	2.700	1.350
Rmerge	0.193	0.130	0.370
Rmeas	0.215	0.142	0.426
Total number of observations	1981
Number of reflections	405	56	151
<I/σ(I)>	3.77	7.6	2.05
Completeness [%]	68.6	63.6	71.9
Redundancy	4.891	6.036	4.04
CC(1/2)	0.980	0.992	0.948

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	10% ethanol