6UOE

3-25 Fab germline-reversion variant bound to an HCMV gB-derived peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	80
Detector technology	IMAGE PLATE
Collection date	2019-09-09
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54178
Spacegroup name	C 1 2 1
Unit cell lengths	118.663, 67.587, 84.031
Unit cell angles	90.00, 133.12, 90.00

Refinement procedure

Resolution	33.319 - 1.800
R-factor	0.1617
Rwork	0.160
R-free	0.18580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB entries 6BLA & 6DDM
Data reduction software	MOSFLM (7.2.2)
Data scaling software	Aimless (1.11.19)
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.320	1.840
High resolution limit [Å]	1.800	1.800
Number of reflections	41589	2525
<I/σ(I)>	10.2	3
Completeness [%]	92.1	95.5
Redundancy	2.5	2.3
CC(1/2)	0.995	0.910

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	0.1 M magnesium chloride, 13.4% PEG3350, 16.75% PEG400, 0.1 M Tris, pH 8.5