6UNX

Structure of E. coli FtsZ(L178E)-GTP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-24
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	37.316, 85.142, 41.274
Unit cell angles	90.00, 107.03, 90.00

Refinement procedure

Resolution	42.571 - 1.400
R-factor	0.184
Rwork	0.183
R-free	0.20330
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6umk
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.571	1.435
High resolution limit [Å]	1.400	1.400
Rpim	0.025	0.105
Number of reflections	41849	1322
<I/σ(I)>	15.1	4.1
Completeness [%]	86.3
Redundancy	3.5
CC(1/2)	0.998	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	Peg 8000, Cacodylate buffer, 0.15 M calcium acetate