6UN9

Crystal Structure of the Q7VLF5_HAEDU protein from Haemophilus ducreyi. Northeast Structural Genomics Consortium Target Hdr25

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-12
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 65 2 2
Unit cell lengths	70.609, 70.609, 504.039
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.440 - 2.800
R-factor	0.3032
Rwork	0.296
R-free	0.37340
Structure solution method	SAD
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXDE
Refinement software	PHENIX (1.17rc5_3630)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	60.000	60.000	2.900
High resolution limit [Å]	2.800	6.030	2.800
Rmerge	0.171	0.061	0.909
Rmeas	0.173	0.062	0.931
Rpim	0.031	0.010	0.186
Number of reflections	33685	2286	1509
<I/σ(I)>	4.1
Completeness [%]	97.6	99.6	80.2
Redundancy	34.6	32.4	19.9
CC(1/2)		1.000	0.930

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH		291	2.0 M Ammonium sulfate