6UN8

Wild type ANT bound to neomycin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2017-10-01
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	56.661, 97.794, 102.231
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.650
R-factor	0.1743
Rwork	0.174
R-free	0.19780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1kny
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.710
High resolution limit [Å]	1.649	1.649
Rmeas	0.061	0.615
Number of reflections	68631	6808
<I/σ(I)>	24.2
Completeness [%]	99.2	99.8
Redundancy	4.3	4
CC(1/2)		0.931

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	16-17% PEG 8K, 0.1 M Tris pH 7-8, 0.1-0.2 M MgCl2