6UN7
Crystal structure of green fluorescent protein (GFP); S65T, Y66(3-OMeY); ih circular permutant (50-51)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.883, 68.601, 60.186 |
| Unit cell angles | 90.00, 101.64, 90.00 |
Refinement procedure
| Resolution | 36.140 - 1.500 |
| R-factor | 0.168 |
| Rwork | 0.167 |
| R-free | 0.19100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ofk |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13RC2_2986) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.141 | 1.540 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.056 | 2.716 |
| Number of reflections | 63500 | 4542 |
| <I/σ(I)> | 19.66 | 1.19 |
| Completeness [%] | 97.8 | 95.9 |
| Redundancy | 13.32 | 13.08 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.15 M ammonium acetate, 34% PEG 3350 |
