6UN5

Crystal structure of green fluorescent protein (GFP); S65T, Y66(2,3,5-F3Y); ih circular permutant (50-51)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	48.394, 68.464, 60.596
Unit cell angles	90.00, 102.07, 90.00

Refinement procedure

Resolution	38.930 - 1.360
R-factor	0.175
Rwork	0.174
R-free	0.20500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6ofk
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.13RC2_2986)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	38.929	1.400
High resolution limit [Å]	1.358	1.360
Rmerge	0.045	1.945
Number of reflections	81547	5968
<I/σ(I)>	24.13	1.31
Completeness [%]	98.2	97.2
Redundancy	13.36	13.23

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.15 M ammonium acetate, 31% PEG 3350