6UMR
Structure of DUF89 - D291A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-19 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.03 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 91.260, 194.691, 114.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.490 - 2.210 |
| R-factor | 0.222 |
| Rwork | 0.221 |
| R-free | 0.25660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pt1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.071 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.990 | 2.289 |
| High resolution limit [Å] | 2.210 | 2.210 |
| Rmerge | 0.134 | 0.484 |
| Number of reflections | 50993 | 4984 |
| <I/σ(I)> | 8.1 | |
| Completeness [%] | 98.7 | |
| Redundancy | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277.15 | 18% PEG 4000, 200mM MgCl2, 100mM Tris-HCl pH 8.5 |
