6UMQ
Structure of DUF89
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-04-08 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.11 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 90.140, 194.473, 114.201 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.490 - 1.850 |
| R-factor | 0.1971 |
| Rwork | 0.196 |
| R-free | 0.22400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pt1 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.215 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.490 | 1.916 |
| High resolution limit [Å] | 1.840 | 1.850 |
| Rmerge | 0.118 | 0.215 |
| Number of reflections | 85552 | 8480 |
| <I/σ(I)> | 8.4 | |
| Completeness [%] | 98.5 | 99.27 |
| Redundancy | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277.15 | 18% PEG 4000, 200mM MgCl2, 100mM Tris-HCl pH 8.5 |
