6UMK

Structure of E. coli FtsZ(L178E)-GDP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-23
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	37.427, 85.189, 41.398
Unit cell angles	90.00, 106.92, 90.00

Refinement procedure

Resolution	35.915 - 1.350
R-factor	0.1869
Rwork	0.186
R-free	0.20450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vaw
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.915	1.380
High resolution limit [Å]	1.350	1.350
Rmeas	0.035
Rpim	0.021	0.047
Number of reflections	52607	3192
<I/σ(I)>	23.8	4.7
Completeness [%]	96.7
Redundancy	3
CC(1/2)	0.998	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	Peg 8000, cacodylate pH 6.5, Calcium acetate