6UMJ

Crystal structure of erenumab Fab-c

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2014-08-12
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	P 32 2 1
Unit cell lengths	73.481, 73.481, 177.167
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.700
R-factor	0.2269
Rwork	0.225
R-free	0.26710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.836
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.800
High resolution limit [Å]	2.700	5.800	2.700
Rmerge	0.171	0.127	0.744
Rmeas	0.176	0.131	0.761
Rpim	0.038	0.030	0.161
Total number of observations	336957
Number of reflections	15692	1723	1506
<I/σ(I)>	7.1
Completeness [%]	98.9	99.9	98
Redundancy	21.5	19.7	21.9
CC(1/2)		0.987	0.950

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	277	0.1 M sodium acetate, pH 5.0, 30% MPD, 5% 1,3-butanediol