6UMH

Crystal structure of erenumab Fab-a

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2014-08-06
Detector	RIGAKU SATURN 92
Wavelength(s)	1.5418
Spacegroup name	C 1 2 1
Unit cell lengths	124.273, 55.913, 170.086
Unit cell angles	90.00, 110.93, 90.00

Refinement procedure

Resolution	30.000 - 2.150
R-factor	0.1869
Rwork	0.185
R-free	0.22380
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.217
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.230
High resolution limit [Å]	2.150	4.630	2.150
Rmerge	0.068	0.042	0.557
Rmeas	0.073	0.045	0.629
Rpim	0.027	0.017	0.284
Total number of observations	400151
Number of reflections	58318	6155	4831
<I/σ(I)>	11.2		2.38
Completeness [%]	97.8	100	81.9
Redundancy	6.9	7.2	4.6
CC(1/2)		0.999	0.855

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	0.2 M ammonium dihydrogen phosphate, 0.1 M Tris, pH 8.5, 50% MPD, 4% 1,3-propanediol