6UKK
Crystal Structure of a Domain-swapped Fluorogen Activating Protein DiB3 Dimer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-02-17 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97857 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 89.618, 38.993, 51.837 |
Unit cell angles | 90.00, 113.21, 90.00 |
Refinement procedure
Resolution | 30.940 - 1.600 |
R-factor | 0.1847 |
Rwork | 0.184 |
R-free | 0.19920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qwd |
RMSD bond length | 0.011 |
RMSD bond angle | 1.721 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | MOLREP (11.6.02) |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.940 | 1.630 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmeas | 0.046 | 0.517 |
Rpim | 0.021 | 0.282 |
Total number of observations | 93957 | |
Number of reflections | 21731 | 1012 |
<I/σ(I)> | 13.4 | 1.8 |
Completeness [%] | 98.9 | 90.2 |
Redundancy | 4.3 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 294 | 0.8 M sodium citrate, 50 mM sodium borate, 0.1 sodium acetate, pH 3.5 |