6UKK
Crystal Structure of a Domain-swapped Fluorogen Activating Protein DiB3 Dimer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-02-17 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97857 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 89.618, 38.993, 51.837 |
| Unit cell angles | 90.00, 113.21, 90.00 |
Refinement procedure
| Resolution | 30.940 - 1.600 |
| R-factor | 0.1847 |
| Rwork | 0.184 |
| R-free | 0.19920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qwd |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.721 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP (11.6.02) |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.940 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmeas | 0.046 | 0.517 |
| Rpim | 0.021 | 0.282 |
| Total number of observations | 93957 | |
| Number of reflections | 21731 | 1012 |
| <I/σ(I)> | 13.4 | 1.8 |
| Completeness [%] | 98.9 | 90.2 |
| Redundancy | 4.3 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 3.5 | 294 | 0.8 M sodium citrate, 50 mM sodium borate, 0.1 sodium acetate, pH 3.5 |
