6UKF

HhaI endonuclease in Complex with DNA at 1 Angstrom Resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.8000
Spacegroup name	P 1 21 1
Unit cell lengths	61.018, 37.317, 69.027
Unit cell angles	90.00, 109.77, 90.00

Refinement procedure

Resolution	26.390 - 1.000
R-factor	0.1549
Rwork	0.155
R-free	0.16620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6uke
RMSD bond length	0.004
RMSD bond angle	0.825
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.400	1.010
High resolution limit [Å]	1.000	1.000
Rmerge	0.104	1.069
Rpim	0.033	0.588
Number of reflections	148223	5385
<I/σ(I)>	16.3	0.9
Completeness [%]	93.9
Redundancy	8.7
CC(1/2)		0.378

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	292	35% v/v 2-propanol, 0.1 M sodium phosphate dibasic / citric acid, pH 4.2