6UKF
HhaI endonuclease in Complex with DNA at 1 Angstrom Resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-26 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.8000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.018, 37.317, 69.027 |
| Unit cell angles | 90.00, 109.77, 90.00 |
Refinement procedure
| Resolution | 26.390 - 1.000 |
| R-factor | 0.1549 |
| Rwork | 0.155 |
| R-free | 0.16620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6uke |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.825 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.400 | 1.010 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.104 | 1.069 |
| Rpim | 0.033 | 0.588 |
| Number of reflections | 148223 | 5385 |
| <I/σ(I)> | 16.3 | 0.9 |
| Completeness [%] | 93.9 | |
| Redundancy | 8.7 | |
| CC(1/2) | 0.378 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.2 | 292 | 35% v/v 2-propanol, 0.1 M sodium phosphate dibasic / citric acid, pH 4.2 |
