6UIH

Crystal structure of the core domain from the GST-like protein GDAP1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97931
Spacegroup name	I 4 3 2
Unit cell lengths	196.598, 196.598, 196.598
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.149 - 2.826
R-factor	0.2121
Rwork	0.211
R-free	0.22350
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	Aimless (0.7.1)
Phasing software	AutoSol
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	98.300	98.300	2.980
High resolution limit [Å]	2.826	8.940	2.830
Rmerge	0.107	0.054	1.986
Rmeas	0.108	0.054	1.999
Rpim	0.013	0.007	0.228
Total number of observations		35248	172164
Number of reflections	15877	586	2265
<I/σ(I)>	37.3	92.8	2.9
Completeness [%]	100.0	99.9	100
Redundancy	70.1	60.2	76
CC(1/2)	1.000	1.000	0.912

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	298	1.0M Citrate, 200mM NaCl, 10mM TCEP