6UIE
Structure of the cytoplasmic domain of the T3SS sorting platform protein PscK from P. aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.722, 81.733, 96.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.900 - 2.550 |
| R-factor | 0.2388 |
| Rwork | 0.236 |
| R-free | 0.29360 |
| Structure solution method | SAD |
| Starting model (for MR) | 4a0e |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | CRANK2 |
| Refinement software | PHENIX (1.17rc2_3615) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.900 | 48.900 | 2.660 |
| High resolution limit [Å] | 2.550 | 8.830 | 2.550 |
| Rmerge | 0.260 | 0.093 | 2.008 |
| Total number of observations | 24461 | 117347 | |
| Number of reflections | 28173 | 435 | 1823 |
| <I/σ(I)> | 15 | 35 | 3.3 |
| Completeness [%] | 100.0 | 99.8 | 100 |
| Redundancy | 63 | 56.2 | 64.4 |
| CC(1/2) | 0.999 | 0.999 | 0.976 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 20% (w/v) PEG 1500, 100 mM Tris, 100 mM ammonium sulfate |
