6UI5
Tmn9 in complex with cofactor FAD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-26 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.976255 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.205, 85.605, 207.683 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.788 - 2.400 |
R-factor | 0.205 |
Rwork | 0.203 |
R-free | 0.24870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5xgv |
RMSD bond length | 0.010 |
RMSD bond angle | 1.244 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.27) |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.790 | 49.790 | 2.330 |
High resolution limit [Å] | 2.260 | 9.310 | 2.260 |
Rmerge | 0.078 | 0.033 | 1.338 |
Rmeas | 0.085 | 0.036 | 1.466 |
Rpim | 0.034 | 0.015 | 0.589 |
Total number of observations | 328949 | 4603 | 25638 |
Number of reflections | 52087 | 860 | 4307 |
<I/σ(I)> | 12 | 36.4 | 1.3 |
Completeness [%] | 99.6 | 98.9 | 96.6 |
Redundancy | 6.3 | 5.4 | 6 |
CC(1/2) | 0.996 | 0.992 | 0.524 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 30% w/v PEG 8000, 100 mM sodium cacodylate pH 6,5, 200 mM sodium acetate |