6UHQ

Crystal Structure of C148 mGFP-cDNA-3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2018-12-01
Detector	RAYONIX MX-300
Wavelength(s)	0.97872
Spacegroup name	C 1 2 1
Unit cell lengths	106.630, 50.580, 56.690
Unit cell angles	90.00, 110.33, 90.00

Refinement procedure

Resolution	50.040 - 2.850
R-factor	0.1883
Rwork	0.181
R-free	0.27390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5n9o
RMSD bond length	0.007
RMSD bond angle	1.748
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	53.159	53.159	3.000
High resolution limit [Å]	2.850	9.010	2.850
Rmerge		0.054	0.677
Rmeas	0.212	0.066	0.802
Rpim	0.112	0.037	0.421
Total number of observations	21886
Number of reflections	6723	230	976
<I/σ(I)>	5.8	11.4	2.1
Completeness [%]	99.3	98.9	99.2
Redundancy	3.3	3.1	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	295	1 microliter C148 mGFP-cDNA-3 (5 mg/mL (protein concentration) in 10 mM Tris Buffer pH 7.4, 137 mM NaCl) + 1 microliter crystallization condition (0.1 M MES pH 6.5, 30% (w/v) PEG 4000) in a sitting drop with a 70 microliter reservoir (0.1 M MES pH 6.5, 30% (w/v) PEG 4000)