6UHP

Crystal Structure of C148 mGFP-ncDNA-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-10-03
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.97857
Spacegroup name	P 1 21 1
Unit cell lengths	59.053, 51.605, 100.409
Unit cell angles	90.00, 106.97, 90.00

Refinement procedure

Resolution	56.480 - 2.900
R-factor	0.3411
Rwork	0.340
R-free	0.37280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5n9o
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	96.037	56.482	3.060
High resolution limit [Å]	2.900	9.170	2.900
Rmerge		0.060	0.657
Rmeas	0.165	0.067	0.730
Rpim	0.073	0.030	0.311
Total number of observations	66203
Number of reflections	13070	444	1895
<I/σ(I)>	7.9	14.5	2.8
Completeness [%]	99.8	98.4	100
Redundancy	5.1	4.4	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	1 microliter C148 mGFP-ncDNA-1 (5 mg/mL (protein concentration) in 10 mM Tris Buffer pH 7.4, 137 mM NaCl) + 1 microliter crystallization condition (0.2 M sodium chloride, 0.1 M Bis-Tris Buffer pH 5.5, 25% (w/v) PEG 3350) in a sitting drop with a 70 microliter reservoir (0.2 M sodium chloride, 0.1 M Bis-Tris Buffer pH 5.5, 25% (w/v) PEG 3350)