6UHL
Crystal Structure of C148 mGFP-scDNA-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-07-03 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 64.870, 52.290, 86.800 |
| Unit cell angles | 90.00, 94.13, 90.00 |
Refinement procedure
| Resolution | 43.290 - 1.910 |
| R-factor | 0.2304 |
| Rwork | 0.228 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4eul |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.776 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 43.290 | 43.290 | 1.960 |
| High resolution limit [Å] | 1.910 | 8.540 | 1.910 |
| Rmerge | 0.122 | 0.051 | 1.313 |
| Rmeas | 0.128 | 0.054 | 1.444 |
| Rpim | 0.040 | 0.017 | 0.579 |
| Total number of observations | 435564 | ||
| Number of reflections | 43945 | 547 | 2202 |
| <I/σ(I)> | 12.1 | 29.4 | 1.3 |
| Completeness [%] | 96.9 | 98.6 | 67.2 |
| Redundancy | 9.9 | 9.2 | 5.8 |
| CC(1/2) | 0.998 | 0.998 | 0.492 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | 1 microliter C148 mGFP-scDNA-1 (5 mg/mL (protein concentration) in 10 mM Tris Buffer pH 7.4, 137 mM NaCl) + 1 microliter crystallization condition (0.1 M SPG Buffer pH 8, 25% (w/v) PEG 1500) in a sitting drop with a 70 microliter reservoir (0.1 M SPG Buffer pH 8, 25% (w/v) PEG 1500) |
