6UHJ

X-ray Structure of C148 mGFP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-10-03
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.97857
Spacegroup name	P 21 21 21
Unit cell lengths	51.510, 62.900, 69.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.610 - 1.500
R-factor	0.1546
Rwork	0.153
R-free	0.18520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5n9o
RMSD bond length	0.013
RMSD bond angle	1.871
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	51.510	51.510	1.580
High resolution limit [Å]	1.500	4.750	1.500
Rmerge		0.043	0.347
Rmeas	0.094	0.049	0.399
Rpim	0.043	0.023	0.192
Number of reflections	36338	1291	5176
<I/σ(I)>	10.7	18.7	3.6
Completeness [%]	98.8	99.7	98
Redundancy	4.5	4.3	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	295	1 microliter C148 mGFP (5 mg/mL of protein in 10 mM Tris Buffer pH 7.4, 137 mM NaCl) + 1 microliter crystallization condition (0.1 M sodium acetate, 25% (w/v) PEG 4000, 8% (v/v) isopropanol) in a sitting drop with a 70 microliter reservoir (0.1 M sodium acetate, 25% (w/v) PEG 4000, 8% (v/v) isopropanol)