6UHB

Crystal Structure of Human RYR Receptor 3 (848-1055)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-06-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97927
Spacegroup name	C 2 2 21
Unit cell lengths	84.695, 88.444, 145.206
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.780 - 2.504
R-factor	0.2401
Rwork	0.234
R-free	0.27630
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.616
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	HKL-3000
Refinement software	PHENIX (1.11.1-2575_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.540
High resolution limit [Å]	2.500	6.780	2.500
Rmerge	0.116	0.100	0.697
Rmeas	0.126	0.110	0.765
Rpim	0.050	0.045	0.311
Total number of observations	120407
Number of reflections	18807	1019	892
<I/σ(I)>	6.4
Completeness [%]	99.6	99	95.9
Redundancy	6.4	6.2	5.6
CC(1/2)		0.980	0.782

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	297	0.1 M Na2HPO4:Citric Acid pH 4.2, 1.6 M NaH2PO4/0.4 M K2HPO4