6UHA

Open-form Crystal Structure of Human RYR Receptor 3 ( 848-1055)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-03-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97927
Spacegroup name	I 41
Unit cell lengths	94.495, 94.495, 116.895
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.248 - 2.855
R-factor	0.2248
Rwork	0.223
R-free	0.26780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4rev
RMSD bond length	0.005
RMSD bond angle	0.901
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	HKL-3000
Refinement software	PHENIX (1.11.1-2575_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.900
High resolution limit [Å]	2.850	7.730	2.850
Rmerge	0.062	0.044	0.668
Rmeas	0.075	0.053	0.834
Rpim	0.042	0.029	0.491
Total number of observations	31797
Number of reflections	11458	548	570
<I/σ(I)>	7.2
Completeness [%]	96.0	87.8	98.8
Redundancy	2.8	3	2.6
CC(1/2)		0.970	0.498

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	297	PEG3350 15%, protein in co-crystallization with 30mM Caffeine and 10mM ATP