6UHA
Open-form Crystal Structure of Human RYR Receptor 3 ( 848-1055)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-03-23 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97927 |
| Spacegroup name | I 41 |
| Unit cell lengths | 94.495, 94.495, 116.895 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.248 - 2.855 |
| R-factor | 0.2248 |
| Rwork | 0.223 |
| R-free | 0.26780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4rev |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.901 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.11.1-2575_1309) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.900 |
| High resolution limit [Å] | 2.850 | 7.730 | 2.850 |
| Rmerge | 0.062 | 0.044 | 0.668 |
| Rmeas | 0.075 | 0.053 | 0.834 |
| Rpim | 0.042 | 0.029 | 0.491 |
| Total number of observations | 31797 | ||
| Number of reflections | 11458 | 548 | 570 |
| <I/σ(I)> | 7.2 | ||
| Completeness [%] | 96.0 | 87.8 | 98.8 |
| Redundancy | 2.8 | 3 | 2.6 |
| CC(1/2) | 0.970 | 0.498 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 297 | PEG3350 15%, protein in co-crystallization with 30mM Caffeine and 10mM ATP |
