6UH9
Crystal structure of DAD2 D166A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-06-20 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9537 |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.690, 56.530, 68.040 |
| Unit cell angles | 94.47, 94.67, 108.83 |
Refinement procedure
| Resolution | 30.330 - 1.520 |
| R-factor | 0.1724 |
| Rwork | 0.171 |
| R-free | 0.19910 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dnp |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.513 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.6.2) |
| Phasing software | PHASER (2.8.2) |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.330 | 30.310 | 1.550 |
| High resolution limit [Å] | 1.520 | 8.190 | 1.520 |
| Rmerge | 0.089 | 0.036 | 0.669 |
| Rmeas | 0.104 | 0.043 | 0.775 |
| Rpim | 0.052 | 0.023 | 0.390 |
| Total number of observations | 1601 | 14673 | |
| Number of reflections | 75017 | 465 | 3744 |
| <I/σ(I)> | 7.8 | 22.9 | 1.6 |
| Completeness [%] | 95.1 | 91.2 | 93.2 |
| Redundancy | 3.9 | 3.4 | 3.9 |
| CC(1/2) | 0.997 | 0.997 | 0.721 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1 M Trizma-acetate-bicine (pH 8.5), 0.06 M divalents (MgCl2 and CaCl2), 45-55% PEG 500 MME PEG 20000 |
