6UH3
Crystal structure of bacterial heliorhodopsin 48C12
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-11 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.449, 102.139, 56.770 |
| Unit cell angles | 90.00, 94.04, 90.00 |
Refinement procedure
| Resolution | 49.000 - 2.700 |
| R-factor | 0.2652 |
| Rwork | 0.264 |
| R-free | 0.27930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3am6 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.830 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 14449 | 1858 |
| <I/σ(I)> | 4.8 | |
| Completeness [%] | 95.9 | 91 |
| Redundancy | 3.3 | 3.1 |
| CC(1/2) | 0.942 | 0.310 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 5.4 | 273 | 50 mM sodium citrate tribasic dihydrate, pH 5.4, 350 mM potassium citrate tribasic monohydrate, 37% PEG400, 0.5% w/v DDM |
