6UGL
Vibrio cholerae quorum-sensing receptor VqmA bound to its autoinducer ligand DPO
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X17B1 |
| Synchrotron site | NSLS |
| Beamline | X17B1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-04-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.459, 84.680, 116.042 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 28.860 - 2.030 |
| R-factor | 0.2171 |
| Rwork | 0.213 |
| R-free | 0.27730 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.764 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXS |
| Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.900 | 2.100 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmeas | 0.158 | 0.780 |
| Number of reflections | 32521 | 2905 |
| <I/σ(I)> | 12.56 | |
| Completeness [%] | 98.7 | |
| Redundancy | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 298 | 0.1 M Tris pH 7.3, 16% PEG3350 and 0.2 M sodium malonate |
