6UGJ

Crystal structure of a fragment of E. coli tRNA(Asp) consisting of its acceptor stem/T stem-loop. Short unit cell.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-01
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97620
Spacegroup name	H 3 2
Unit cell lengths	43.845, 43.845, 259.285
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.250 - 1.600
R-factor	0.2165
Rwork	0.214
R-free	0.27240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5 base pair A-form RNA helix
RMSD bond length	0.009
RMSD bond angle	1.615
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.250	1.691
High resolution limit [Å]	1.600	1.600
Rmerge	0.156	4.054
Rmeas	0.160	4.200
Rpim	0.034	1.089
Number of reflections	9777	6865
<I/σ(I)>	11.5	1
Completeness [%]	92.0	68.8
Redundancy	21.7	14.3
CC(1/2)	0.999	0.492

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		287	2 M ammonium sulfate