6UEY

Pistol ribozyme transition-state analog vanadate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 42 21 2
Unit cell lengths	91.829, 91.829, 123.444
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.652 - 2.800
R-factor	0.2157
Rwork	0.212
R-free	0.25150
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.900	2.850
High resolution limit [Å]	2.800	2.800
Rpim		0.017
Number of reflections	13611	675
<I/σ(I)>	37.9
Completeness [%]	99.8
Redundancy	10.2
CC(1/2)	0.987

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.1 M sodium acetate, pH 4.6, 0.02 M calcium chloride, 25% MPD