6UEQ

Structure of TBP bound to C-C mismatch containing TATA site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-03-24
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1
Spacegroup name	P 2 2 21
Unit cell lengths	45.403, 45.583, 155.231
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	77.615 - 2.400
R-factor	0.2155
Rwork	0.212
R-free	0.24870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6uep
RMSD bond length	0.003
RMSD bond angle	0.568
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	155.200	2.496
High resolution limit [Å]	2.400	2.400
Rpim	0.035	0.245
Number of reflections	12803	1255
<I/σ(I)>	10.3	2
Completeness [%]	96.4
Redundancy	7
CC(1/2)	0.998	0.896

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	sodium acetate pH 4.5, 28% PEG 400