6UEI

Crystal structure of human zinc finger antiviral protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-14
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0782
Spacegroup name	P 65
Unit cell lengths	124.350, 124.350, 43.040
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.970 - 2.510
R-factor	0.208
Rwork	0.206
R-free	0.24400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3u9g
RMSD bond length	0.010
RMSD bond angle	1.090
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.040	43.040	2.660
High resolution limit [Å]	2.510	7.420	2.510
Rmerge	0.084	0.044	1.340
Rmeas	0.089	0.047	1.413
Total number of observations	133707
Number of reflections	13247	550	2098
<I/σ(I)>	15.54	47.51	1.57
Completeness [%]	99.9	99.3	99.7
Redundancy	10.093	9.304	10.005
CC(1/2)	0.999	0.998	0.873

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293.15	20% Peg 3350 and 0.2M Sodium Malonate