6UCT

Crystal structure of Mal de Rio Cuarto virus P9-1 viroplasm protein (C-arm deletion mutant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-17
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.980
Spacegroup name	P 43 21 2
Unit cell lengths	86.557, 86.557, 95.598
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.280 - 3.470
R-factor	0.223
Rwork	0.220
R-free	0.27700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3vjj
RMSD bond length	0.009
RMSD bond angle	1.040
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.800	3.800
High resolution limit [Å]	3.470	3.470
Rmerge	0.132	2.912
Rmeas	0.136	3.013
Rpim	0.027	0.582
Number of reflections	5035	1138
<I/σ(I)>	15.8	1.6
Completeness [%]	99.2	96.8
Redundancy	24.9	25.6
CC(1/2)	0.999	0.548

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	13% (w/v) PEG 8000, 0.2 M calcium acetate