6UCM

Transcription factor DeltaFosB bZIP domain self-assembly, type-II crystal

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-08-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.987
Spacegroup name	P 32 2 1
Unit cell lengths	101.925, 101.925, 51.347
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.384 - 2.424
R-factor	0.212
Rwork	0.208
R-free	0.24490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vpe
RMSD bond length	0.005
RMSD bond angle	0.577
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.470
High resolution limit [Å]	2.424	6.590	2.430
Rmerge	0.086	0.032	1.448
Rmeas	0.091	0.034	1.531
Rpim	0.028	0.011	0.495
Number of reflections	11940	649	580
<I/σ(I)>	9
Completeness [%]	100.0	100	100
Redundancy	10.5	9.5	9.8
CC(1/2)		0.998	0.638

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	2 M ammonium sulfate, 5% (v/v) isopropanol