6UC9
Guanine riboswitch bound to O6-cyclohexylmethyl guanine
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-20 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.5406 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 133.468, 35.171, 41.889 |
Unit cell angles | 90.00, 91.55, 90.00 |
Refinement procedure
Resolution | 19.860 - 1.941 |
R-factor | 0.1893 |
Rwork | 0.187 |
R-free | 0.21110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fe5 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14-3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 19.860 | 2.011 |
High resolution limit [Å] | 1.941 | 1.941 |
Rmerge | 0.158 | 0.445 |
Rmeas | 0.171 | 0.513 |
Rpim | 0.064 | 0.248 |
Number of reflections | 14355 | 1226 |
<I/σ(I)> | 11.96 | 2.085 |
Completeness [%] | 97.9 | 85.26 |
Redundancy | 6.6 | 3.8 |
CC(1/2) | 0.588 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 297 | 10 mM potassium HEPES pH 7.5, 22.6% v/v PEG 3000 MW, 35-45 mM cobalt hexamine, and 600-650 mM ammonium acetate |