6UC8

Guanine riboswitch bound to 8-aminoguanine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-03
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.5406
Spacegroup name	C 1 2 1
Unit cell lengths	132.545, 35.184, 41.934
Unit cell angles	90.00, 90.34, 90.00

Refinement procedure

Resolution	19.956 - 1.898
R-factor	0.1909
Rwork	0.187
R-free	0.22420
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fe5
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.14-3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.960	1.966
High resolution limit [Å]	1.898	1.898
Rmerge	0.073	0.228
Rmeas	0.083	0.300
Rpim	0.040	0.193
Number of reflections	13646	572
<I/σ(I)>	16.75	1.506
Completeness [%]	87.6	36.89
Redundancy	3.7	1.5
CC(1/2)		0.900

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	297	10 mM potassium HEPES pH 7.5, 20% v/v PEG 3000 MW, 35-47 mM cobalt hexamine, and 600 mM ammonium acetate