6UC8
Guanine riboswitch bound to 8-aminoguanine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-03 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.5406 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 132.545, 35.184, 41.934 |
| Unit cell angles | 90.00, 90.34, 90.00 |
Refinement procedure
| Resolution | 19.956 - 1.898 |
| R-factor | 0.1909 |
| Rwork | 0.187 |
| R-free | 0.22420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fe5 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14-3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.960 | 1.966 |
| High resolution limit [Å] | 1.898 | 1.898 |
| Rmerge | 0.073 | 0.228 |
| Rmeas | 0.083 | 0.300 |
| Rpim | 0.040 | 0.193 |
| Number of reflections | 13646 | 572 |
| <I/σ(I)> | 16.75 | 1.506 |
| Completeness [%] | 87.6 | 36.89 |
| Redundancy | 3.7 | 1.5 |
| CC(1/2) | 0.900 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 297 | 10 mM potassium HEPES pH 7.5, 20% v/v PEG 3000 MW, 35-47 mM cobalt hexamine, and 600 mM ammonium acetate |
