6UC7

Structure of guanine riboswitch bound to N2-acetyl guanine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-12-03
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.5406
Spacegroup name	C 1 2 1
Unit cell lengths	132.107, 35.082, 41.606
Unit cell angles	90.00, 90.99, 90.00

Refinement procedure

Resolution	19.740 - 1.798
R-factor	0.1837
R-free	0.20930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fe5
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.14-3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.740	1.862
High resolution limit [Å]	1.798	1.798
Rmerge	0.066	0.182
Rmeas	0.079	0.232
Rpim		0.142
Number of reflections	16940	1007
<I/σ(I)>	19.3	3.05
Completeness [%]	93.8	56.89
Redundancy	3	1.9
CC(1/2)		0.903

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		297	10 mM potassium HEPES pH 7.5, 20% v/v PEG 3000 MW, 30 mM cobalt hexamine, 600 mM ammonium acetate.