6UBU
1.60 A resolution structure of the guanine riboswitch bound to guanine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-25 |
| Detector | DECTRIS PILATUS 200K |
| Wavelength(s) | 1.5406 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 132.175, 35.260, 42.276 |
| Unit cell angles | 90.00, 90.86, 90.00 |
Refinement procedure
| Resolution | 19.660 - 1.600 |
| R-factor | 0.2022 |
| Rwork | 0.200 |
| R-free | 0.22000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fe5 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.022 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14-3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.660 | 1.654 |
| High resolution limit [Å] | 1.597 | 1.597 |
| Rmerge | 0.361 | |
| Rmeas | 0.131 | |
| Rpim | 0.061 | |
| Number of reflections | 37435 | 593 |
| <I/σ(I)> | 13.67 | 1.876 |
| Completeness [%] | 83.4 | 23.15 |
| Redundancy | 4.5 | 1 |
| CC(1/2) | 0.622 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 297 | 10 mM potassium HEPES pH 7.5, 20% v/v PEG 3000 MW, 40-50 mM cobalt hexamine, 600-650 mM ammonium acetate |
