6UBO
Fluorogen Activating Protein Dib1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-01-01 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 43 |
Unit cell lengths | 44.843, 44.843, 158.235 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.730 - 1.580 |
R-factor | 0.1644 |
Rwork | 0.163 |
R-free | 0.20250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qwd |
RMSD bond length | 0.023 |
RMSD bond angle | 2.394 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.640 |
High resolution limit [Å] | 1.580 | 3.400 | 1.580 |
Rmerge | 0.051 | 0.025 | 0.469 |
Rmeas | 0.054 | 0.026 | 0.504 |
Rpim | 0.020 | 0.010 | 0.183 |
Total number of observations | 321104 | ||
Number of reflections | 42279 | 4314 | 4181 |
<I/σ(I)> | 11.4 | ||
Completeness [%] | 99.0 | 99.5 | 97.9 |
Redundancy | 7.6 | 7.6 | 7.4 |
CC(1/2) | 1.000 | 0.893 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.1M Na citrate pH 5.0, 30% PEG 550MME, 0.2M NaSCN |