6UBH
Structure of the MM7 Erbin PDZ variant in complex with a high-affinity peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2018-02-06 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.000, 39.010, 58.380 |
| Unit cell angles | 72.55, 72.61, 96.06 |
Refinement procedure
| Resolution | 36.770 - 1.800 |
| R-factor | 0.2102 |
| Rwork | 0.197 |
| R-free | 0.22510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6UBG |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.021 | 0.053 |
| Rmeas | 0.290 | 0.071 |
| Rpim | 0.019 | 0.048 |
| Number of reflections | 26382 | 6175 |
| <I/σ(I)> | 26.7 | 12.9 |
| Completeness [%] | 78.3 | 58.7 |
| Redundancy | 2.2 | 2.1 |
| CC(1/2) | 0.999 | 0.991 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 298 | 7% PEG8K, 100 mM Sodium Acetate pH 4.5 |
