6UBC

Crystal structure of a GH128 (subgroup VII) oligosaccharide-binding protein from Cryptococcus neoformans (CnGH128_VII)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979460
Spacegroup name	I 2 3
Unit cell lengths	118.410, 118.410, 118.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.390 - 1.650
R-factor	0.1721
Rwork	0.170
R-free	0.20750
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.826
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.390	1.750
High resolution limit [Å]	1.650	1.650
Number of reflections	33192	5316
<I/σ(I)>	16.65
Completeness [%]	99.8
Redundancy	40.1
CC(1/2)	0.999	0.589

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	291	Sodium acetate 0.1 M ammonium sulfate 2.0 M