6UBB

Crystal structure of a GH128 (subgroup VI) exo-beta-1,3-glucanase from Aureobasidium namibiae (AnGH128_VI) with laminaribiose at the surface-binding site

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-12
Detector	DECTRIS PILATUS3 2M
Wavelength(s)	1.458570
Spacegroup name	P 21 21 21
Unit cell lengths	121.349, 152.733, 169.135
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.300 - 2.350
Rwork	0.265
R-free	0.30100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	1.193
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0253)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.490
High resolution limit [Å]	2.350	2.350
Number of reflections	130821	20840
<I/σ(I)>	4.8
Completeness [%]	99.9
Redundancy	6.7
CC(1/2)	0.980	0.433

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.18 M tri-ammonium citrate, 18% v/v PEG 3,350, 0.01 M Cobalt (II) chloride