6UB7

Crystal structure of a GH128 (subgroup V) exo-beta-1,3-glucanase from Cryptococcus neoformans (CnGH128_V)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-12
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.458
Spacegroup name	P 21 21 21
Unit cell lengths	58.896, 60.066, 96.085
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.280 - 1.800
R-factor	0.1865
Rwork	0.185
R-free	0.21760
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.869
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.020	1.910
High resolution limit [Å]	1.800	1.800
Number of reflections	31515	4570
<I/σ(I)>	13.47
Completeness [%]	98.0
Redundancy	4.56
CC(1/2)	0.999	0.691

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	sodium/potassium phosphate 2.4 M 20% glycerol