6UB0
Crystal structure of a GH128 (subgroup III) curdlan-specific exo-beta-1,3-glucanase from Blastomyces gilchristii (BgGH128_III) in complex with laminaribiose at -2 and -1 subsites
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-17 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.45855 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.561, 34.231, 56.351 |
| Unit cell angles | 90.00, 104.88, 90.00 |
Refinement procedure
| Resolution | 32.110 - 1.750 |
| R-factor | 0.2226 |
| Rwork | 0.220 |
| R-free | 0.26470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6uay |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.958 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.110 | 1.860 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 18575 | 2873 |
| <I/σ(I)> | 24.65 | 4.2 |
| Completeness [%] | 98.9 | 96.2 |
| Redundancy | 4.61 | 4.14 |
| CC(1/2) | 0.999 | 0.976 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.05 M Potassium di-hydrogen phosphate 24% Polyethylene glycol 8,000 |
