6UAZ

Crystal structure of a GH128 (subgroup III) curdlan-specific exo-beta-1,3-glucanase from Blastomyces gilchristii (BgGH128_III) in complex with glucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-01
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.458540
Spacegroup name	P 1
Unit cell lengths	36.460, 48.928, 57.757
Unit cell angles	102.97, 89.34, 93.76

Refinement procedure

Resolution	41.200 - 1.850
R-factor	0.1666
Rwork	0.164
R-free	0.21680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.820
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.200	1.960
High resolution limit [Å]	1.850	1.850
Number of reflections	31291	4890
<I/σ(I)>	11.51
Completeness [%]	94.2
Redundancy	3.42
CC(1/2)	0.997	0.717

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.05 M Potassium di-hydrogen phosphate 24% Polyethylene glycol 8,000