6UAZ
Crystal structure of a GH128 (subgroup III) curdlan-specific exo-beta-1,3-glucanase from Blastomyces gilchristii (BgGH128_III) in complex with glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | LNLS BEAMLINE W01B-MX2 |
| Synchrotron site | LNLS |
| Beamline | W01B-MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-01 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.458540 |
| Spacegroup name | P 1 |
| Unit cell lengths | 36.460, 48.928, 57.757 |
| Unit cell angles | 102.97, 89.34, 93.76 |
Refinement procedure
| Resolution | 41.200 - 1.850 |
| R-factor | 0.1666 |
| Rwork | 0.164 |
| R-free | 0.21680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.820 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.200 | 1.960 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 31291 | 4890 |
| <I/σ(I)> | 11.51 | |
| Completeness [%] | 94.2 | |
| Redundancy | 3.42 | |
| CC(1/2) | 0.997 | 0.717 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.05 M Potassium di-hydrogen phosphate 24% Polyethylene glycol 8,000 |
