6UAX
Crystal structure of a GH128 (subgroup II) endo-beta-1,3-glucanase from Sorangium cellulosum (ScGH128_II)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-05-15 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 0.979460 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 46.756, 65.628, 86.551 |
| Unit cell angles | 90.00, 95.96, 90.00 |
Refinement procedure
| Resolution | 46.550 - 1.300 |
| R-factor | 0.1309 |
| Rwork | 0.129 |
| R-free | 0.16720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6uaq |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.077 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.550 | 1.390 |
| High resolution limit [Å] | 1.300 | 1.310 |
| Number of reflections | 123123 | 18875 |
| <I/σ(I)> | 14.91 | 2.93 |
| Completeness [%] | 97.7 | 93.1 |
| Redundancy | 6.59 | 6.56 |
| CC(1/2) | 0.999 | 0.858 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 18% PEG3350 4% isopropanol 0.1 M calcium chloride 0.1 M HEPES |
