6UAV
Crystal structure of a GH128 (subgroup II) endo-beta-1,3-glucanase from Pseudomonas viridiflava (PvGH128_II)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | LNLS BEAMLINE W01B-MX2 |
Synchrotron site | LNLS |
Beamline | W01B-MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-10 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 1.239860 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 87.663, 33.648, 84.259 |
Unit cell angles | 90.00, 113.06, 90.00 |
Refinement procedure
Resolution | 40.330 - 1.500 |
R-factor | 0.17224 |
Rwork | 0.171 |
R-free | 0.20034 |
Structure solution method | SIRAS |
RMSD bond length | 0.014 |
RMSD bond angle | 1.817 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELXDE |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.450 | 1.590 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.091 | 0.844 |
Rmeas | 0.100 | 0.941 |
Number of reflections | 36444 | 5775 |
<I/σ(I)> | 12.19 | 1.74 |
Completeness [%] | 99.2 | 98.1 |
Redundancy | 5.38 | 5.06 |
CC(1/2) | 0.999 | 0.702 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 291 | ammonium sulfate 2 M bis-tris 0.1 M |